Fundamentals of Ultrasound: Acoustic Properties of Human Tissues

Most human organs can be examined using medical ultrasound, including common examples such as superficial organs (thyroid, breast, salivary glands, superficial lymph nodes, etc.), the heart and blood vessels, abdominal organs (liver, spleen, gallbladder, pancreas, adrenal glands, etc.), the genitourinary system (kidneys, ureters, bladder, uterus, ovaries, scrotum, testes, etc.), skin and muscle tissues, brain tissue, and more. To understand how different tissues and organs appear on ultrasound imaging, let’s explore the details.

01

Internationally, the intensity of human tissue reflection echoes is classified into four grades: high echo, isoecho, hypoecho, and anechoic. For more detailed description, it can also be further divided into high echo, relatively high echo, isoecho, hypoecho, relatively hypoecho, and anechoic. High echo accompanied by a distinct acoustic shadow posteriorly may also be referred to as strong echo.

1. Hyperechoic: High echogenicity is produced by bones, calculi, gas, fibrous connective tissue, etc. A acoustic shadow is often present behind bones and calculi. 

2. Isoechoic: The echogenicity intensity of normal liver parenchyma is defined as isoechoic.

3. Hypoechoic: The echogenicity intensity is lower than that of normal liver parenchyma. 

4. Anechoic: Normal human blood, urine, etc., appear as anechoic, and pathological changes such as effusions, cysts, abscesses, etc., also appear as anechoic.

SonoMaxx Handheld Ultrasound MX6 for Thyroid and Thyroid Cyst Scanning

Overall, liquid structures appear as anechoic dark areas, while solid structures exhibit varying degrees of echogenicity. Homogeneous solid structures present as uniformly hypoechoic or isoechoic. Heterogeneous structures demonstrate mixed echogenicity. Calcified or gas-containing structures exhibit extremely strong echogenicity with posterior acoustic shadows.

It should be noted that the terms “hyperechoic,” “isoechoic,” and “hypoechoic” in sonographic imaging are relative. In most cases, these classifications are determined by comparison with the echogenicity of the observed tissue or organ. Additionally, they are influenced by the performance and settings of the imaging equipment, such as probe frequency, dynamic range, gain, and the use of tissue harmonics.

02

During the propagation of ultrasound, sound energy absorption and attenuation occur. The more water content in human tissues, the lower the sound attenuation; the higher the content of protein and calcium, the greater the sound attenuation, and the faster the sound velocity propagates within tissues, the higher the sound attenuation; the more protein components in body fluids, the higher the sound attenuation.

The sound attenuation of different tissues in human body is as follows: bone, calcification, stone> scar, cartilage, tendon> liver, kidney, muscle, brain> fat, blood> urine, bile, cyst fluid, pleural effusion, ascites.

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Bones, calcifications, calculi, and gas-filled lungs exhibit strong echogenicity with posterior acoustic shadows. However, certain calculi and calcified structures, such as small nodules and minor calcifications, may lack acoustic shadows due to factors including ultrasound frequency, focal point, and incident angle.

Fibrous tissue, which constitutes the organ capsule, vascular walls, peripheral nerves, tendons, etc. with high echogenicity, but the echo intensity is low when propagating in the uniformally arranged fibrous tissue. The difference between high echo and strong echo is that there is no acoustic shadow behind.

Solid organs such as the liver, kidney, spleen, and pancreas exhibit isoechoic properties, with variations in echo intensity among different solid organs. 

Fat is hypoechoic, but when the fat tissue forms an interface with other tissues, it appears hyperechoic. Urine, bile, cyst fluid, pleural effusion, and ascites (transudate) are anechoic.

SonoMaxx Handheld Ultrasound MX6 for Bladder Scanning

The acoustic properties of pathological tissues can be classified into liquid, solid, calcified, and gas. The acoustic characteristics of the same disease may vary at different stages of its course, with corresponding differences in echogenicity.

Simple inflammatory edema may exhibit hypoechoic characteristics due to increased fluid content and relative reduction in tissue components. Fibrosis of liver tissue or intracellular fat infiltration can result in hyperechoic manifestations. Calculi and calcifications demonstrate the strongest echogenicity, followed by fibrosis, while large scar tissue may show hypoechoic patterns. Small hepatic hemangiomas and renal angiomysocarcinomas typically present as hyperechoic. In contrast, typical lymphomas exhibit the weakest echogenicity, sometimes approaching anechoic. However, high-frequency probes may reveal distinct hypoechoic features when scanning lymphomas.
Familiarity with anatomical knowledge and mastery of the display patterns of echo intensity and quantity in organs and lesions facilitate accurate disease diagnosis.

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